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Check FAQAbout Mario
Freelance VIDEO JOURNALIST & FILMMAKER based in Rome, with personal 4K video equipment, digital editing workstation and wireless audio recording system. Since 2008 I've been freelancing for international broadcasters and private companies, producing, filming and editing more than 200 documentaries, reports, insight features and corporate videos. Among my past and current clients: RUPTLY (Germany), China Plus News (China), Russia Today (Russia), PressTV (Iran), MedPage Today (USA), Global News (Canada), JoVE (USA), Scarlett Entertainment (UK), Ten TV Network (Egypt) and Channel 9 (Israel). - 100% Easy-going & reliable - Excellent English speaking / writing skills - 24/7 Availability on call - Will travel wherever needed if asked to E q u i p m e n t: CAMERA Panasonic Lumix DC-GH5 [4K & Full HD] LENSES Laowa 7,5mm f/2.0 RECTILINEAR [Lite Version] Panasonic Lumix G 25mm f/1.7 ASPH. Panasonic Lumix G 42,5mm f/1.7 ASPH. / POWER O.I.S. Panasonic LUMIX G VARIO 14-140mm / F3.5-5.6 ASPH. / POWER O.I.S. ACTION CAM & CAMCORDERS Akaso V50 X Action Cam [4K & Full HD] Sony XDCAM PMW-EX1R [Full HD] Sony HDR-CXH10VE [Full HD] WIRELESS AUDIO RECORDING Sennheiser SK 100 body-pack transmitter Sennheiser SKM 100 hand-held microphone transmitter Sennheiser EK 100 diversity receiver Sennheiser ME 2 clip-on microphone DIRECTIONAL MICROPHONES Sony ECM-672 Røde Video Micro
English
Spanish
Italian
Video Package (Web / Broadcast)
Audio package (Radio / Podcast)
Interview (Video / Broadcast)
Portfolio
Renzi and Obama metting // VIDEO REPORT
Banksy expo in Rome // VIDEO REPORT
FEMEN shove the Holy Cross up their... // VIDEO REPORT
Rome March held by Fascists // VIDEO REPORT
Costa Concordia rotation begins // VIDEO REPORTS
Nailympics in Rome // VIDEO REPORT
Rome hit by anti austerity riots // VIDEO REPORT
Zoo animals cool off with ice cream and watermelon // VIDEO REPORT
Russian Ball in Rome // VIDEO REPORT
Femen steal Baby Jesus at Nativity scene in Vatican // VIDEO REPORT
Bvlgari: Anna Foglietta // SOCIAL MEDIA AD
Bvlgari: Sabine Getty // SOCIAL MEDIA AD
Bvlgari: Isabella Ferrari // SOCIAL MEDIA AD
Coronavirus Outbreak in Italy // MINI DOC
My China Story: Luisa Paladini // MINI DOC
My China Story: Gianni Catani // MINI DOC
Mario Corrado // SHOWREEL
On the Way to China: Mortara, Italy // MINI DOC
#iAsk President - Italy // VIDEO REPORT
RT // LIVE INTERVIEW
RAN World // CORPORATE VIDEO
Adipso // INSTITUTIONAL VIDEO
Sea Train // COMMERCIAL
Platges // DOC FILM
MyVisto // COMMERCIAL
The Other Dalí // DOC TEASER
Complementary oligonucleotides regulate induced fit ligand binding in duplexed aptamers
Duplexed aptamers (DAs) engineered by hybridizing an aptamer-complementary element (ACE) to an aptamer can actively bind a ligand from the duplex state through an ACE-regulated induced fit mechanism. Using an ATP DNA aptamer and a solution-based equilibrium assay, DAs exhibited affinities significantly higher than predicted by conformational selection alone. Different ACEs regulated the level of induced fit binding and cooperative allostery of the DA. A non-equilibrium surface-based assay validated these findings, indicating that ACEs regulate ATP DA ligand binding dynamics, opening new avenues for the study and design of ligand-responsive nucleic acids.
Complementary oligonucleotides regulate induced fit ligand binding in duplexed aptamers
Duplexed aptamers (DAs) engineered by hybridizing an aptamer-complementary element (ACE) to an aptamer can actively bind a ligand from the duplex state through an ACE-regulated induced fit mechanism. Using an ATP DNA aptamer and a solution-based equilibrium assay, DAs exhibited affinities significantly higher than predicted by conformational selection alone. Different ACEs regulated the level of induced fit binding and cooperative allostery of the DA. A non-equilibrium surface-based assay validated these findings, indicating that ACEs regulate ATP DA ligand binding dynamics, opening new avenues for the study and design of ligand-responsive nucleic acids.
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